FIG 2.

Experimental scheme. Total protein harvested from fresh B. bacteriovorus HD100 AP cells and axenically replicating B. bacteriovorus HD100 M1.1 HI mutant cells was investigated by CCMS. Of 3,584 encoded proteins (23), 777 were detected by LC-MS/MS, but only 84 proteins presented competitive cyclic di-GMP specific binding. These binders demonstrated c-di-GMP binding exclusive to the AP (blue circle), unique to the HI mutants (green circle), or in both (red circle). AP-specific and common binders represent cyclic di-GMP effectors functional in the AP. HI-specific binders represent cyclic di-GMP effectors functional in the GP. The functionality of shared binders in the GP cannot be determined due to possible deregulated protein production in HI mutants (15).