TABLE 2.
Plasmids used in this work
| Plasmid | Description | Reference or source |
|---|---|---|
| pBJ114 | Kanr galK | 81 |
| pSWU30 | Tetr | 42 |
| pSW105 | PpilA Kanr | 44 |
| pBGS18 | Kanr | 82 |
| pET24b(+) | Kanr, expression vector | Novagen |
| pET28a(+) | Kanr, expression vector | Novagen |
| pTP110 | pSW105 PA5295WT-Strep-tag II Kanr | This study |
| pTP112 | pSW105 PA5295E328A-Strep-tag II Kanr | This study |
| pTP114 | pSW105 dgcAWT-Strep-tag II Kanr | This study |
| pTP131 | pSW105 dgcAD164A-Strep-tag II Kanr | This study |
| pTP120 | pBJ114, in-frame deletion construct MXAN1525 | This study |
| pTP125 | pBJ114, in-frame deletion construct MXAN2643 | This study |
| pNGS010 | pBJ114, in-frame deletion construct MXAN2997 | This study |
| pTP126 | pBJ114, in-frame deletion construct dmxA | This study |
| pDJS01 | pBJ114, in-frame deletion construct MXAN3735 | This study |
| pTP127 | pBJ114, in-frame deletion construct MXAN4029 | This study |
| pIH01 | pBJ114, in-frame deletion construct MXAN4463 | This study |
| pTP121 | pBJ114, in-frame deletion construct MXAN5199 | This study |
| pDJS02 | pBJ114, in-frame deletion construct MXAN5366 | This study |
| pIH03 | pBJ114, in-frame deletion construct MXAN5791 | This study |
| pDJS03 | pBJ114, in-frame deletion construct MXAN7362 | This study |
| pJH01 | pBJ114, in-frame deletion construct actA | This study |
| pTP122 | pBJ114, in-frame deletion construct MXAN4257 | This study |
| pTP123 | pBJ114, in-frame deletion construct tmoK | This study |
| pNGS009 | pBJ114, in-frame deletion construct MXAN5053 | This study |
| pNGS007 | pBJ114, in-frame deletion construct MXAN5340 | This study |
| pTP105 | pBJ114, in-frame deletion construct MXAN2424 | This study |
| pTP106 | pBJ114, in-frame deletion construct MXAN2530 | This study |
| pTP116 | pBJ114, in-frame deletion construct MXAN2061 | This study |
| pTP128 | pBJ114, in-frame deletion construct MXAN2807 | This study |
| pTP124 | pBJ114, in-frame deletion construct MXAN4232 | This study |
| pTP117 | pBJ114, in-frame deletion construct MXAN4675 | This study |
| pNGS008 | pBJ114, in-frame deletion construct MXAN6098 | This study |
| pTP133 | pBGS18, 500-bp fragment internal to dmxA | This study |
| pTP140 | pSWU30 Pnat-dmxA Tetr | This study |
| pDJS57 | pSWU30 Pnat-tmoK Tetr | This study |
| pDJS31 | pET24b(+) dgcAWT Kanr | This study |
| pTP33 | pET28a(+) sgmT Kanr | 13 |
| pTP137 | pET28a(+) dmxA223–722 Kanr | This study |
| pDJS53 | pET24b(+) tmoK654–1109 Kanr | This study |