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Diguanylate cyclase expression in Dictyostelium. The promoter of the gene encoding the Dictyostelium DgcA was fused to the E. coli lacZ reporter gene and transformed into Dictyostelium cells. Staining of fruiting structures with 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) shows that DgcA is expressed in prestalk and stalk cells. Bar, 100 μm. The image was previously published (67) and is reprinted with permission of the publisher.
