TABLE 2.
Primers used in this study for PCR
| Primer no. | Primer name | Sequencea |
|---|---|---|
| ANG1579 | F-NcoI-FL-KdpD | GGGCCATGGCAAACACTGAATCGCTAAACATAGG |
| ANG1580 | R-EcoRI-FL-KdpD | GGGGAATTCACGTCTCCTTCGTTAAAGTCTG |
| ANG1581 | R-EcoRI-KdpD-NT | GGGGAATTCGCGAAACGTTTGCCTTTAGGACGATAG |
| ANG1584 | F-NcoI-KdpD-CT | GGGCCATGGCCATTACTAAAAAGCAACTTTATCG |
| ANG1775 | R-EcoRI-KdpD-KdpD | GGGGAATTCGGTTCTTTATCACTCATCAAGTCGGC |
| ANG1776 | F-NcoI-KdpD-USP | GGGCCATGGAAAAAGTCCGACACAACCATAAAACGTC |
| ANG1777 | 1F-NcoI-FL-KdpD2 | GGGCCATGGAAAGTACATATAAAAAAAGAGGGAAACTT |
| ANG1778 | 2R-NcoImut-KdpD2 | GACATCGATACCGTGCGATAGAATTTCTTCTATATCC |
| ANG1779 | 3F-NcoImut-KdpD2 | GAAATTCTATCGCACGGTATCGATGTCTGGACAAC |
| ANG1780 | 4R-EcoRI-FL-KdpD2 | GGGGAATTCGGACGACCAATAATGTTGTTTTCATCCA |
| ANG1811 | F-NheI-KdpD(USP) | GGGGCTAGCGAAAAAGTCCGACACAACCATAAAACGTC |
| ANG1812 | R-HindIII-KdpD(USP) | GGGAAGCTTTCAGAAACGTTTGCCTTTAGGACGATAGGG |
| ANG2053 | F-USPmutS244A | CACTCAAACCTCATATTGCTGTGGCAATTGCTGGGAGCATTTATAA |
| ANG2054 | R-USPmutS244A | TTATAAATGCTCCCAGCAATTGCCACAGCAATATGAGGTTTGAGTG |
| ANG2055 | F-USPmutY271A | CCTGTTTTTTTCGAATACATCTATAGCAATAGCAGTGAACTTCGCATGTTCTT |
| ANG2056 | R-USPmutY271A | AAGAACATGCGAAGTTCACTGCTATTGCTATAGATGTATTCGAAAAAAACAGG |
a
Restriction sites in primer sequences are underlined.