Table 1. Primers for qPCR.
| App. | Species | gene | Forward start | Forward primer (5’-3’) | Backward start | Reverse primer (5’-3’) |
|---|---|---|---|---|---|---|
| RT | mouse | Gapdh | CATCTTCTTGTGCAGTGCCAG | GGCAACAATCTCCACTTTGCC | ||
| RT | mouse | Actb | CCTTCAACACCCCAGCCATGTACG | GGCACAGTGTGGGTGACCCCGTC | ||
| RT | mouse | Rbbp5 | TGGACAGAACTACCCAGAGGA | CCATCGTTACAGCCAACAGC | ||
| RT | mouse | Ash2l | TGACACCTTTGGAATAGACACG | AAGGCACTAAGGCACATTTCTT | ||
| RT | mouse | Wdr5 | GTCTCAGCCGTTCATTTCAACC | CGAAGGACACTGGAGGATTGT | ||
| RT | mouse | Dpy30 | ACCCTCACTCTGAGTACGGG | GGACTGTAGATCCACCTTCTGT | ||
| RT | mouse | Set1a | TGCTGTCCCTCGTAGACTGG | GGCTCTTTCCGTTTTACCTTGA | ||
| RT | mouse | Set1b | GTGAAGTCCGGTGAGCACAA | CAGGAGGCGATTCGGTCTTTG | ||
| RT | mouse | Mll1 | GCAGATTGTAAGACGGCGAG | GAGAGGGGGTGTTCCTTCCTT | ||
| RT | mouse | Mll2 | GATGAGAATGGCTCGTTGTGG | TCTATCTTGTCACACTTCCGGTA | ||
| RT | mouse | Mll3 | TCAGTGCCATTCAAGGGTCC | ACCCATGAGTGGATGGTGAGA | ||
| RT | mouse | Mll4 | GAGGACTCGCTCATGTCCCT | GCGGAGATAGGTGTGGCTC | ||
| RT | mouse | Oct4 | ACATCGCCAATCAGCTTGG | AGAACCATACTCGAACCACATCC | ||
| RT | mouse | Sox2 | ACAGATGCAACCGATGCACC | TGGAGTTGTACTGCAGGGCG | ||
| RT | mouse | Klf4 | GCACACCTGCGAACTCACAC | CCGTCCCAGTCACAGTGGTAA | ||
| RT | mouse | Myc | CCACCAGCAGCGACTCTGA | TGCCTCTTCTCCACAGACACC | ||
| RT | IRES | AACAGACCTTGCATTCCTTTGGCG | TAAGGCCGGTGTGCGTTTGTCTAT | |||
| RT | GFP | TCTTGTAGTTGCCGTCGTCCTTGA | TGACCCTGAAGTTCATCTGCACCA | |||
| RT | mouse | Nanog | CCTCCAGCAGATGCAAGAACTC | CTTCAACCACTGGTTTTTCTGCC | ||
| ChIP | human | Wdr5 P1 | TSS -266 | AGGGCACACTAGCACTTTCCTGTA | TSS -187 | TTGACTGGTGCAGGCCCTTCTAAT |
| ChIP | human | Wdr5 P2 | TSS +391 | ACTGGGTCCTCTTTCCCGCA | TSS +492 | ACTGAGTGGCTTGGTGGGTCT |
| ChIP | human | Wdr5 dst | Chr 9: 134,211,630 | AGCTCCATGCCACTGCCTTACTTA | Chr 9: 134,211,824 | TGCCTTCTTTCTCCACAGCAGCTA |
| ChIP | human | Dpy30 | TSS -332 | TGAGGAAGTTCAGTTGGTCGAGGA | TSS -231 | ACCAAGTGACCAGCCCAGTTAGAA |
| ChIP | mouse | Cad | TSS -388 | GCCATGTCGCAGCCAAGAAGATTT | TSS -307 | CAATGGCCGCTTCAGCCTTAAACA |
| ChIP | mouse | Wdr5 P1 | TSS -254 | ACCAAGAGGTTTCCCAACAGTCCT | TSS -92 | ACTGGTGTTCGGTAACTGCAGACT |
| ChIP | mouse | Wdr5 P2 | TSS +373 | CACTGGGAGTGGAATTCTCTG | TSS +486 | CTAGGACACAAATGAGGGTTGT |
| ChIP | mouse | Dpy30 | TSS -596 | TCACAAGCTTGAACCATCACCCAG | TSS -494 | TCTGCCTCCCAAGTGCTGGAATTA |
| ChIP | mouse | Dpy30 | TSS +280 | ACTGTGAACCCAGAGGTTGTGCTA | TSS +359 | AGGTGTATGAGAAGGGTTGAGCCA |
| ChIP | mouse | Dpy30 | TSS +10244 | TCTTGGGCAGTAACTGTAGCAGCA | AAGTGCTTGCCAGTACTCAGCTCT | |
| ChIP | mouse | Dpy30 | TSS +23114 | CCGTGCTTCCCACAAAGCAAATGT | CATGCCACCAGCAACATTAGCACA | |
| ChIP | mouse | Intergenic | Chr8: 72,806,101 | AAGGGGCCTCTGCTTAAAAA | Chr8: 72,806,240 | AGAGCTCCATGGCAGGTAGA |
| ChIP | mouse | Fgf4 | TSS +681 | ACCGGTAGACAGGAGATGAG | TSS +769 | ACTCTAAGCCTCTTGGGATCT |
| ChIP | mouse | Irf6 | Ref. (15) | GAGGGAGGACAGACACCTGA | GCCGTCCCAAAACTACTTGA | |
| ChIP | mouse | Cdh1 | Ref. (15) | CCGAGCTCAGTGTTTGCTC | CAGGACCCTCCACATACCTG | |
| ChIP | mouse | Sall4 | Ref. (61) | AACCTGCATTCTCCTACAGACC | TTTCTTTAATGCCTGCATTTTG | |
| ChIP | mouse | Oct4-A | Ref. (61) | GACGGCAGATGCATAACAAA | AGGAAGGGCTAGGACGAGAG | |
| ChIP | mouse | Oct4-B | Ref. (61) | TGTGAACTTGGCGGCTTC | CCTCCACTCTGTCATGCTCA | |
| ChIP | mouse | Postn | Ref. (15) | TATGCTCTGCTGCTGCTGTT | AACAAGCCAGGGACTTACCC |