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. Author manuscript; available in PMC: 2015 Dec 21.
Published in final edited form as: Biochem Biophys Res Commun. 2015 May 19;463(3):216–221. doi: 10.1016/j.bbrc.2015.05.026

Fig. 1.

Fig. 1

CDζ CAR-transduced CD8+ T cells are susceptible to HIV-1 infection. A) CD8+ T cells were transduced with EGFP-P2A-CDζ (CDζ) or control EGFP (EGFP) vector. The cells transduced with the vector were sorted based on their EGFP expression and infected with one of three different HIV-1 strains, HIV-1NL4-3, HIV-1NFNSX SL9 or HIV-1JR-CSF at MOI = 0.2, 1.0 or 1.0, respectively. Cells were plated at 1 × 105 cells/500 ml in a 48-well plate, and HIV-1 p24Gag production in the culture supernatants was determined by ELISA at day 7 post infection. CD4+ T cells transduced with control EGFP were used as a positive control for HIV-1 infection. B) Intracellular HIV-1 p24Gag staining by KC57 antibody. CD8+ T cells transduced with EGFP-P2A-CDζ (CDζ) or control EGFP (EGFP) vector were infected with HIV-1NL4-3, HIV-1NFNSX SL9 or HIV-1JR-CSF. Intracellular HIV-1 p24Gag was analyzed by fiow cytometry after staining PE-conjugated KC57 antibody 7 days post infection.