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. Author manuscript; available in PMC: 2015 Dec 21.
Published in final edited form as: Sci Transl Med. 2015 May 13;7(287):287ra69. doi: 10.1126/scitranslmed.aaa6593

Fig. 2. Stem cell transplantation from irradiated mice reveals a defect in short- and long-term repopulating ability.

Fig. 2

(A) Total number of HSCs (LKS+ cells) per hind limb measured by FACS. Data are individual animals, and horizontal bars are means. (B) Schematic to describe transplantation (Tx) of HSCs or unfractionated whole bone marrow (WBM) into lethally irradiated recipients. BM, bone marrow. (C) Representative FACS profiles of stained bone marrow from control and irradiated donor mice used to sort HSCs for transplant. Individual profiles show total scatter, lineage− (lin−), and LKS+ gates. FSC, forward-scattered light; SSC, side-scattered light. (D) Donor cell engraftment in peripheral blood of recipients transplanted with either HSCs or WBM. Total leukocyte engraftment at 1 and 4 months posttransplant is shown. Data are means ± SEM (n = 5 per group for HSC, n = 4 per group for WBM). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s., not significant; compared to control (0 Gy) mice; one-way ANOVA followed by Tukey’s test.