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. Author manuscript; available in PMC: 2015 Dec 21.
Published in final edited form as: Adv Biol Chem. 2015 Jun;5:179–188. doi: 10.4236/abc.2015.54014

Figure 1.

Figure 1

(a) Detection of prepore from Cry toxins using anti polyclonal antisera obtained from Bravo, A, et al. [1] at 1:50,000 dilution. Lane 1 = Prepore from Cry1Abwt produced in BtHD1–19. Lane 2 = Prepore from Cry1Ab F371C mutant produced in Bt4Q7. Lane 3 = Prepore from Cry1Aa wt produced in Bt sotto 4E3. Lane 4 = Cry1Ab produced in E. coli. Lane 5 = Cry1Aa produced in E. coli; (b) Detection of cadherin association in purified prepore of Cry1Ab. Monomer and prepore obtained were treated with anti Bt-R1 antibody at 1:10,000 dilution of the primary antibody. Lane 1 = Purified Cry1Ab monomer toxin (200 ng), Lane 2 = Purified Cry1Ab prepore toxin (50 ng).