Figure 1.

Tumors derived from the transplantation of metastatic lines 66.1, 410.4 and 168 or from nonmetastatic lines 410 and 67 were harvested. The tissue lysates were prepared and were immunoblotted with cyclooxygenase-2 antibody followed by horseradish peroxidase-conjugated secondary antibodies, and the expression was compared with 72 kDa Cox-2 protein standard (STD).