Fig 4. Kinetic of expression of a representative gene of ergosterol pathway in Paracoccidioides submitted to hypoxia.

The kinetic of expression of Pberg3, representative gene of ergosterol pathway, was analysed using qRT-PCR. Pb01 yeast cells were submitted to normoxia and hypoxia for 2, 4 and 6 h at 37°C in BHI medium and total RNAs were extracted. Molecules of cDNA were synthesized and used for qRT-PCR. The data were normalized using the constitutive gene encoding the alpha tubulin as the endogenous control. Data are expressed as the mean ± standard deviation of the triplicates of independent experiments. *, significantly different from the normoxic condition (experimental control), at a p value of P ≤ 0.01. The accession number to erg3 is PAAG_03651, from Paracoccidioides genome database (http://www.broadinstitute.org/annotation/genome/paracoccidioides_brasiliensis/MultiHome.html).