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. 2015 Dec 21;10(12):e0144922. doi: 10.1371/journal.pone.0144922

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© 2015 Xie et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — Sf9 cells were transfected with plasmids expressing LvPT-V5 (V5-tagged LvPT) and LvPT^WSP -V5 (LvPT-V5 without signal peptide). At 6 h after heat shock, the culture medium, supernatant and precipitate of cell lysates were harvested. After separation by SDS-PAGE, LvPT-V5 and LvPT^WSP -V5 were detected by western blot using anti-His antibody as a probe. Asterisks indicate the LvPT-V5 while triangles indicate LvPT^WSP -V5 in samples. Compared with LvPT^WSP-V5, LvPT-V5 could be detected in culture medium and tended to be a secretory protein in Sf9 cells.