Fig 10. Chitin binding assay of LvPT.

LvPT-V5 was incubated with colloidal chitin at 4°C for 2 h, in order to eliminate dissociative proteins, the LvPT-V5-chitin complex washed three times by TBS (50 Mm Tris, pH 8.0) supplemented with 50 mM NaCl, 100 mM NaCl, 200 mM NaCl and 500 mM NaCl, respectively. The LvPT-V5 bind to chitin (bands around 40 kDa) was detected by western blot using anti-His antibody as a probe. BSA was used as negative control and detected by coomassie brilliant blue. Colloidal chitin (protein-free) was used as reference to indicate the bands in negative control were not proteins but polymer of N-acetyl glucosamine.