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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Neurobiol. 2015 Jun 21;53(5):3477–3493. doi: 10.1007/s12035-015-9295-y

Figure 1. Suppression of neuronal death in MMP-9−/− hippocampus.

Figure 1

(A) Hippocampal neurons from wild-type (WTN) and MMP-9−/− (KON) mice plated on astrocytes harvested from WT (WTA) or KO (KOA) mice. Left: Identification of neurons by immunostaining for the dendritic marker MAP2. Representative of each of four culture conditions at DIV17. Right: Change in neuronal density over development in vitro (n=6 cultures, Two-way repeated-measures ANOVA including all 4 culture conditions, F(4,23)=2899; p<0.0001 *p<0.05 KON or KOA vs. WTN or WTA Tukey-Kramer post hoc test. (B) Top: Immunostaining of P14 wild type hippocampus for c-cas3 (red: apoptotic cells), NeuN (green: neurons) and DAPI (blue: nuclei). Bottom: Change in number of c-cas3+ neurons over development in situ (n=4 subjects, One-way repeated-measures ANOVA, F(2,7)=11.5; p=0.015 *p<0.05 versus age-matched WT, Tukey-Kramer post hoc test). (C) Left: Immunostaining of P120 CA1 region of hippocampus for NeuN. Right: Stereological analysis of total number of CA1 stratum pyramidale (SP) neurons (n=6 subjects) at P14 and P120 *p<0.05; Student’s T-test.

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