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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Neurobiol. 2015 Jun 21;53(5):3477–3493. doi: 10.1007/s12035-015-9295-y

Figure 4. Normal dendritic spine density and morphology in MMP-9−/− hippocampal neurons.

Figure 4

(A) Left top: Visualization of dendrites and spines via expression of GFP in cultured WT and MMP-9−/− (KO) hippocampal pyramidal neurons. Dendritic spines (green) colocalize with immunostaining for the pre-synaptic marker VGluT1 (red; DIV17). Left bottom: Mean dendritic spine density (n=15 neurons from 4 cultures, p=0.99; Student’s T-test). Right top: Visualization of dendrites and spines in situ via Golgi staining of WT and MMP-9−/− (KO) CA1 hippocampal pyramidal neurons. Right bottom: Mean dendritic spine density (P14; n=5 neurons from 3 subjects for WT, 7 neurons from 3 subjects for KO, p=0.99, Student’s T-test). (B) Proportion of different spine types: thin spines (spine head diameter<0.6 μm), stubby spines (neck length<0.2 μm), and mushroom spines (spine head diameter>0.6 mm and neck length>0.2 μm) in Golgi-stained WT and MMP-9−/− (KO) hippocampal CA1 pyramidal neurons at P14 (n=5 subjects each, 3 neurons were analyzed per subject, p=0.60, 0.43, 0.30, respectively; Student’s T-test) (C) Distribution of head diameter and neck length of spines analyzed in B. Inset: contribution of each spine type to total (150 spines).

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