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. 2015 Nov;15(3):665–677. doi: 10.1016/j.scr.2015.10.016

Fig. 5.

Fig. 5

Confocal images of GFP-positive clones induced at 12 weeks in CAGG-CreER;R26R-mT/mG mice

(A) Flat-mounted cornea (co), limbus (li) and conjunctiva (cj) from a 12-week old uninjected negative control mouse showing no GFP-positive clones (bv, limbal blood vessels). (B–J) GFP-positive patches and stripes in the corneal epithelium after 3 (B, C, E–J) or 5 (D) tamoxifen injections. (B) 4-Day chase, showing small GFP-positive patches, induced by tamoxifen, and GFP-positive radial stripes, implying leaky expression. (C, D) Radial GFP-positive stripes after 35-week (C) and 21-week (D) chase periods. (E1–4) Images from a confocal Z-stack (8-week chase) showing a GFP-positive stripe in the peripheral cornea connected to GFP-positive cells in the limbus in E4 (arrow). (F-H) GFP-positive patches in the corneal epithelium after 5-day (F), 4-week (G) and 8-week (H) chase periods. (I, J) GFP-positive stripes in the peripheral corneal epithelium (arrows indicate the limbus). Scale bars: 1000 μm (A–D); 100 μm (E); 50 μm (F–J).