Figure 2.

MiR-205 protected renal cells from cisplatin-induced apoptosis

(A) miR-205 was overexpressed by transfection of miR-205 mimics or knockdown by inhibitor against miR-205 as measured by quantitative PCR assay. Scramble served as the control. (B) Representative images of flow cytometry analysis showing Annexin V/PI stained HK-2 cells with scramble, miR-205 mimics or miRNA inhibitor against miR-205, in the presence of cisplatin (100μg/mL) for 6 hr. Results from three independent experiments quantifying the percentage of Annexin V-positive cells are summarized. * P<0.05, and ** P<0.01 compared with the control