Figure 4.

CMTM4 played a role in renal cell apoptosis

(A) Western blot analysis showing the protein levels of CMTM4 in HK-2 cells transfected with CMTM4 overexpression plasmids (OE) (left panel) and siRNA against CMTM4 (right panel). The vector plasmid (Vector) and the scrambled siRNA (NC) served as negative controls, respectively. (B) The result of quantitative analysis from the flow cytometry assay on HK-2 cells transfected with CMTM4 OE plasmid (CMTM4 OE) or siCMTM4 nucleotide fragment (siCMTM4) in the presence of cisplatin (100 μg/ml). * P< 0.05, and ** P<0.01 compared with the control