Figure 5.

Evaluation of apoptosis in K562 cancer cells after co-culture with different concentrations of CuO NPs for 24 hr. A) K562 apoptosis was assessed by acridine orange/propidium staining. The green cells with diffused chromatin are viable, and red cells with condensed chromatin are apoptotic, early apoptosis features were seen after 24 hr, representing intercalated acridine orange (bright green) amongst the fragmented DNA (arrow). B) K562 cell death via apoptosis increased significantly (* P<0.05) in a dose-dependent manner