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. 2015 Dec 22;7:236. doi: 10.3389/fnagi.2015.00236

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Copyright © 2015 Raz, Henseler, Kolk, Riaz, van der Zwaal, Nagels, Nelissen and Raz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological markers of muscle degeneration in torn and non-torn shoulder muscles. Representative images of histological analyses of biopsies of torn infraspinatus (ISp), non-torn RC muscle teres minor (Tmi), and deltoid (Del) in two patients. (A) Gomori-Trichrome staining shows myofibers in blue-green, nuclei in purple. Fat cells are negatively stained (arrow heads) and fibrotic areas are stained light blue-green (asterisks). Scale bar represents 100 μm. (B) Collagen immunostaining (red), nuclei are counterstained with DAPI (blue). Scale bar represents 100 μm. (C) Fatty droplet (red) staining, nuclei are counterstained with DAPI (blue). Scale bar represents 200 μm. (D) Immunostaining for myosin heavy chain (MyHC) isotypes: MyHC-1 (blue), MyHC-2a (red), MyHC-2x (yellow), and the basal lamina (gray). Scale bar represents 100 μm.