Table 1.
Expression of Ralstonia solanacearum popA measured by the β-galactosidase activity of the lacZYA reporter fusion gene in PS medium or PS medium supplemented with plant derived compounds.
| Number | Compounda | β-galactosidase activity (mean ± SD)b | Number | Compounda | β-galactosidase activity (mean ± SD)b |
|---|---|---|---|---|---|
| DMSO | 368 ± 5.2 | DMSO | 194 ± 10.0 | ||
| (1) | Chlorogenic acid | 661 ± 17.2ˆ* | (26) | Synephrine | 224.5 ± 7.5 |
| (2) | Protocatechuic acid | 430 ± 2.5ˆ* | (27) | Curcumol | 185 ± 17.0 |
| (3) | Muscone | 388 ± 21.9 | (28) | Oleanolic acid | 1580 ± 34.5ˆ* |
| (4) | Resveratrol | 467 ± 4.7ˆ* | (29) | Arctigenin | 221 ± 9.5 |
| (5) | Eugenol | 350 ± 1.0 | (30) | Dicoumarolum | 236 ± 19.5 |
| (6) | Gastrodin | 363 ± 3.3 | (31) | Luteolin | 282 ± 62.0 |
| (7) | Cepharanthine | 431 ± 13.4ˆ* | (32) | Esculin hydrate | 275 ± 6.8ˆ* |
| (8) | Deoxyschizandrin | 394 ± 14.5 | (33) | Apigenin | 462 ± 10.0ˆ* |
| (9) | Capsaicin | 434 ± 9.5ˆ* | (34) | Scopolamine butylbromide | 207 ± 7.5 |
| (10) | Icariin | 247 ± 6.1ˆ* | (35) | Diosgenin | 180 ± 7.0 |
| (11) | Tetrandrine | 410 ± 28.0 | (36) | Daphnetin | 204 ± 8.5 |
| (12) | Alantolactone | 362 ± 6.7 | (37) | Stigmasterol | 201 ± 10.5 |
| (13) | Palmitic acid | 284 ± 14.9ˆ* | (38) | Glycyrrhetic acid | 156 ± 2.0ˆ* |
| (14) | Citric acid | 425 ± 52.8 | |||
| DMSO | 186 ± 11.0 | ||||
| DMSO | 310 ± 4.0 | (39) | Succinic acid | 259 ± 5.0ˆ* | |
| (15) | Scopoletin | 452 ± 31.5ˆ* | (40) | Fumaric acid | 277 ± 13.5ˆ* |
| (16) | Tubeimoside | 375 ± 8.5ˆ* | (41) | Stearic acid | 86 ± 9.5ˆ* |
| (17) | Xanthotoxin | 324 ± 24.5 | (42) | L-Malic acid | 396 ± 37.5ˆ* |
| (18) | Bergeninum | 352 ± 4.0ˆ* | (43) | L-Tryptophan | 190 ± 14.5 |
| (19) | Lappaconitine | 314 ± 13.0 | (44) | L-Histidine | 194 ± 10.5 |
| (20) | Taxifolin | 786 ± 28.0ˆ* | (45) | L-valine | 211 ± 16.0 |
| (21) | Podophyllotoxin | 389 ± 3.5ˆ* | (46) | L-Arginine | 212 ± 5.5 |
| (22) | Cryptotanshinone | 344 ± 56.5 | (47) | Benzoic acid | 73 ± 8.5ˆ* |
| (23) | Vindoline | 319 ± 32.0 | (48) | Trans-Cinnamic acid | 164 ± 8.0 |
| (24) | Emodin | 214 ± 12.5ˆ* | (49) | Methyl Jasmonate | 193 ± 9.6 |
| (25) | Patchouli alcohol | 365 ± 24.5 | (50) | Salicylic acid | 212 ± 11.0 |
aPS medium (plant-sucrose medium) was supplemented with DMSO or 100 μM of the indicated compounds. The compounds were assayed two times.
bRalstonia solanacearum strain carrying popA-lacZYA reporter was used in this experiment. The promoter activities of bacteria culture fluid with OD600 of 0.1–0.2 were determined. Values are representative of two independent experiments and three replicates were used for each experiment. Asterisks indicate statistically significantly differences in popA expression between bacterial cells grown in PS medium supplemented with DMSO and PS medium supplemented with different compounds (P < 0.05, Student’s t-test).