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. 2015 Dec 22;9:484. doi: 10.3389/fncel.2015.00484

Figure 1.

Figure 1

Distribution and density of astrocytes in SD and P23H rat retinas. (A–F) Whole-mounted retinas from SD (A,B) and P23H rats (C–F) at P18 (A,C), P120 (D), P360 (E), and P480 (B,F) showing retinal astrocytes (red) at the nerve fiber layer and blood vessels (green). Nuclei were stained with the nuclear marker TO-PRO 3 (blue). Astrocytes have been labeled with antibodies against GFAP. Blood vessels have been labeled with Griffonia simplicifolia isolectin B4. All images were collected from the medial area of the retina. Note the presence of GFAP positive Müller cell end-feet in P23H rats at P120 (D, arrowheads), P360 (E, arrowheads), and P480 (F, arrowheads). Scale bar: 40 μm.