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. 2015 Dec 22;9:484. doi: 10.3389/fncel.2015.00484

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Copyright © 2015 Fernández-Sánchez, Lax, Campello, Pinilla and Cuenca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Müller and cone cell interactions in P23H rat retinas. Whole-mounted retinas from P360 P23H rats showing single (A,B) and double (C,D) staining with γ-transducin (green) and GFAP (red) for labeling cone and reactive Müller cells, respectively. The retinas were flat-mounted with the photoreceptor layer facing up. Note the presence of degenerating cones with short outer segments and axons (A,B, arrowheads). High magnification of a degenerated cone is shown in (B) (lower left corner, inset a). (E,F) Magnification of (B,D) (inset b), respectively, showing cones with normal morphology [E, outer segment (empty arrow), inner segment (empty arrowhead), axons (arrow), and pedicles (arrowhead)] and Müller cell apical processes (red) arranged in parallel to the cones (F). Scale bar: 100 μm (A,C), 10 μm (Inset a), 40 μm (B,D–F).