Table 1.
Primary antibodies and lectins used in this work.
Molecular marker (initials) | Antibody/lectin | Company | Catalog ref. | Working dilution | |
---|---|---|---|---|---|
IHC | WB | ||||
Glial fibrillary acidic protein (GFAP) | Mouse, G-A-5 | Sigma | G 3893 | 1:500 | – |
Glial fibrillary acidic protein (GFAP) | Rabbit, polyclonal | Dako | N1506 | 1:50 | 1:1000 |
Transducin, Gαc subunit (Gt) | Rabbit, polyclonal | Cytosignal | PAB-00801-G | 1:200 | – |
Vimentin | Mouse, V9 | DAKOCytomation | M0725 | 1:100 | – |
Connexin 43 (Cx43) | Rabbit, polyclonal | Sigma | C 6219 | 1:1000 | 1:10000 |
Griffonia simplicifolia IB4 (GS-IB4) | Isolectin IB4 | Invitrogen | I21411 | 1:100 | – |
Collagen type IV | Goat, polyclonal | Millipore | AB769 | 1:100 | – |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Mouse, 6C5 | Millipore | MAB374 | – | 1:10000 |
Primary antibodies and lectins used in this work. The two first columns list the antigen and the animal origin of the antibody. The clone code is specified for monoclonal antibodies. The two central columns provide the commercial company and antibody catalog reference. The two last columns specify the dilution at which each antibody was used for immunohistochemistry (IHC) or immunoblotting (WB).