Figure 1.
(A) ICAM expression obtained by flow cytometry of HUVECs treated with 10 ng/ml TNFalpha at different time points. The expression is quantified by changes in the mean fluorescent channel (MFC). (B) PECAM expression obtained by flow cytometry on HUVECS treated with 10 ng/ml TNFalpha at different time points. Expression units are similar to ICAM (MFC). (C) Differences in total numbers of annexin V+/IP+ cells (late apoptosis) observed via flow cytometry in cultures treated with TNFalpha at different time points. These values are compared with the control culture, in which the number of apoptotic cells is set to 1. (D) Decrease in the number of PCNA+ cells (proliferating cells) in cultures treated with TNFalpha at different time points. *vs. control, #vs. TNFalpha 24 h, § vs. TNFalpha 48 h. **p < 0.05; ***p < 0.001.