Figure 4.

Wound healing assay in mature HUVECs in the presence of treated EPCs. (A) Area free of cells after 24 h of physical damage to the culture. (B) % area closed with respect to the time in which the wound was healing, after contact with treated EPCs on the HUVEC monolayer. (C) Representative images at initial time (upper row) and at the final time point of 24 h (lower row) of wound healing by HUVECs in contact with EPCs treated with concentrations of EMPs from the TNFalpha treatments (40 ×). ^**p < 0.05 vs. Control and 10³ MP/ml; ^#p < 0.001 vs. Control and 10⁵ MP/ml.