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. 2015 Dec 18;47(12):e200. doi: 10.1038/emm.2015.101

Figure 2.

Figure 2

PLD1 regulates the expression of the Rab5 effector, rabaptin-5, through HIF-1α in an oxygen-dependent manner. (a) Quantitative RT–PCR of rabaptin-5 mRNA in HEK293 cells transfected with the indicated constructs. *P<0.05. (b) Quantitative RT–PCR of rabaptin-5 mRNA in HEK293 cells transfected with PLD1 or siRNA for HIF-1α. *P<0.05. (c) ChIP assay of the binding of HIF-1α to the RABEP1 promoter in HEK293 cells transfected with PLD1. (d) Quantitative RT–PCR assay of rabaptin-5 mRNA in HEK293 cells transfected with the indicated constructs under hypoxia or normoxia. Results are shown as the mean±s.d. of the three independent experiments. EGFR, epidermal growth factor receptor; HIF-1α, hypoxia-inducible factor-1α ChIP, chromatin immunoprecipitation; GFP, green fluorescent protein; NS, non-significant; PLD, phospholipase D; RT–PCR, reverse transcription–PCR.