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. 2015 Dec 18;47(12):e200. doi: 10.1038/emm.2015.101

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Copyright © 2015 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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PLD1-PH attenuates tumor progression. (a) RT–PCR assay of rabaptin-5 mRNA in HT29 cells stably transfected with PLD1-PH. (b) HT29 cells expressing empty vector or PLD1-PH cells were xenografted on the left and right flanks of nude mice, respectively (arrows), then analyzed for tumor volume. Photographs show the representative tumors. Data were expressed as the mean±s.d. of seven different mice. (c) Immunoblot analysis of rabaptin-5 in tumor tissues from the xenografted mice injected with vector and PLD1-PH-expressed HT29 cells. Data are representative of three independent experiments. (d) Immunohistochemical staining of rabaptin-5 in the tumor tissues of xenografted mice injected with vector and PLD1-PH-expressed HT29 cells. Data are representative of three independent experiments. PH, pleckstrin homology; PLD, phospholipase D; RT–PCR, reverse transcription–PCR.