Figure 2.

Altered gene expression in FLS co-cultured with HIF-2α-overexpressing chondrocytes. (a) Primary cultures of mouse articular chondrocytes were left untreated or infected with 800 MOI of Ad-C or the indicated MOI of Ad-Epas1 for 24 h. The mRNA levels of the indicated genes were detected by RT-PCR analysis. (b and c) Ad-Epas1-infected chondrocytes incubated on the upper chamber of a transwell plate were co-cultured with primary cultured mouse FLS for 24 h. The expression levels of the indicated genes were determined by RT-PCR (b). Relative mRNA levels (compared to those in untreated cells) were quantified by qRT-PCR (c). Values are presented as mean±s.e.m. (*P<0.005, **P<0.001).