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. 2015 Dec 4;47(12):e197. doi: 10.1038/emm.2015.88

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Copyright © 2015 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Altered gene expression in chondrocytes co-cultured with HIF-2α-overexpressing FLS. (a) Mouse FLS were left untreated or infected with 800 MOI of Ad-C or the indicated MOI of Ad-Epas1 for 24 h. The mRNA levels of the indicated genes were detected by RT-PCR. (b and c) FLS were incubated in the upper chamber of a transwell plate and infected with 800 MOI of Ad-C or the indicated amounts (MOI) of Ad-Epas1, and then incubated with mouse chondrocytes for an additional 24 h. The expression levels of the indicated genes were determined by RT-PCR (b) and quantified by qRT-PCR (c). Values are presented as mean±s.e.m. (*P<0.005).