Table 1. PCR primers and conditions.

Gene	Strand	Primer sequences	Size	At	Origin
Adamts4	S	5′-CATCCGAAACCCTGTCAACTTG-3′	281 bp	58 °C	Mouse
	As	5′-GCCCATCATCTTCCACAATAG-3′
Adamts5	S	5′-GCCATTGTAATAACCCTGCACC-3′	292 bp	58 °C	Mouse
	As	5′-TCAGTCCCATCCGTAACCTTTG-3′
Epas1	S	5′-CGAGAAGAACGACGTGGTGTTC-3′	370 bp	62 °C	Mouse
	As	5′-GTGAAGGCGGGCAGGCTCC-3′
Gapdh	S	5′-TCACTGCCACCCAGAAGAC-3′	450 bp	62 °C	Mouse
	As	5′-TGTAGGCCATGAGGTCCAC-3′
Il6	S	5′-ACCACTCCCAACAGACCTGTCTATACC-3′	435 bp	60 °C	Mouse
	As	5′-CTCCTTCTGTGACTCCAGCTTATCTGTTAG-3′
Nos2	S	5′-TCACTGGGACAGCACAGAAT-3′	510 bp	62 °C	Mouse
	As	5′-TGTGTCTGCAGATGTGCTGA-3′
Mmp2	S	5′-CCAACTACGATGATGAC-3′	233 bp	60 °C	Mouse
	As	5′-ACCAGTGTCAGTATCAG-3′
Mmp3	S	5′-CTGTGTGTGGTTGTGTGCTCATCCTAC-3′	350 bp	58 °C	Mouse
	As	5′-GGCAAATCCGGTGTATAATTCACAATC-3′
Mmp9	S	5′-ACCACATCGAACTTCGA-3′	212 bp	58 °C	Mouse
	As	5′-CGACCATACAGATACTG-3′
Mmp12	S	5′-CCCAGAGGTCAAGATGGATG-3′	482 bp	60 °C	Mouse
	As	5′-GGCTCCATAGAGGGACTGAA-3′
Mmp13	S	5′-TGATGGACCTTCTGGTCTTCTGG-3′	473 bp	55 °C	Mouse
	As	5′-CATCCACATGGTTGGGAAGTTCT-3′
Mmp14	S	5′-GTGCCCTAGGCCTACATCCG-3′	580 bp	55 °C	Mouse
	As	5′-TTGGGTATCCATCCATCACT-3′
Mmp15	S	5′-GAGAGATGTTTGTGTTCAAGGG-3′	260 bp	55 °C	Mouse
	As	5′-TGTGTCAATGCGGTCATAGGG-3′
Ptgs2	S	5′-GGTCTGGTGCCTGGTCTGATGAT-3′	724 bp	65 °C	Mouse
	As	5′-GTCCTTTCAAGGAGAATGGTGC-3′
Tnfa	S	5′-CTTGTCTACTCCCAGGTTCTCTTC-3′	301 bp	58 °C	Mouse
	As	5′-ACAGAGCAATGACTCCAAAGTAGACC-3′

Abbreviations: As, antisense primer; AT, annealing temperature; S, sense primer.