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. 2015 Dec 18;47(12):e199. doi: 10.1038/emm.2015.94

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Copyright © 2015 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Adseverin regulates nuclear factor of activated T cell c1 (NFATc1) expression and nuclear translocation. (a) Protein lysates were prepared from macrophage-colony-stimulating factor (M-CSF)/receptor activator of nuclear factor-κB ligand (RANKL)-treated bone marrow-derived macrophages (BMMs) after small interfering RNA (siRNA) transfection. Western blot analysis was performed using antibodies for c-Fos, NFATc1 and adseverin. (b) After siRNA transfection, BMMs were cultured for 2 days in the presence of M-CSF and RANKL. Nuclear and cytoplasmic fractions were prepared and analyzed using western blot analysis. Lamin B and α-tubulin were used as controls for nuclear and cytoplasmic proteins, respectively. (c) BMMs were transfected with adseverin siRNA and then cultured with M-CSF and RANKL for 3 days. The expression levels of adseverin, NFATc1, tartrate-resistant acid phosphatase (TRAP), cathepsin K, dendritic cell-specific transmembrane protein (DC-STAMP) and v-ATPase were analyzed using real-time PCR. The data are presented as the means±s.d. **P<0.005.