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. 2016 Jan 8;48(1):e202. doi: 10.1038/emm.2015.96

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Aspirin induces interleukin-4 (IL-4) mRNA expression and protein production. (a) HMC-1, EoL-1 and Jurkat cells were treated with various concentrations of aspirin for 4 h. IL-4 expression was measured by quantitative real-time PCR (qPCR); n=4–6. The IL-4 mRNA levels (means±s.e.m.) are expressed relative to the PP1A expression levels (**P<0.01 compared with unstimulated cells at 4 h). (b) Kinetics of IL-4 expression in the presence of 5 mM aspirin; n=2. (c) Cells were transfected with an IL-4 promoter reporter (−649). After 24 h, the transfected cells were stimulated with aspirin for 4 h; n=4 (*P<0.05 and **P<0.01 compared with unstimulated cells at 4 h). (d) Cells were treated with aspirin for 24 h, and the levels of intracellular IL-4 were analyzed by FACS (fluorescence activated cell sorting).