FIGURE 5.

The RT-PCR products were separated in a 2% agarose gel electrophoresis and were stained with ethidium bromide. PCR was performed either in the presence (+) or absence (-) of reverse transcription (RT). (A,B) The PCR products corresponding to Asic1-4 were detected in the B and in the SG. (C) Expression of the housekeeping gene 18S was detected in B and SG. (D) Bar graph showing the expression of Asic1–4 in SG relative to that in B. The expected band sizes (bp) were 99, 169, 142, 116, and 129 for asic1, asic2, asic3, asic4, and 18S, respectively. ^∗200 bp, ⁺100 bp.