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. 2015 Dec 2;6:10116. doi: 10.1038/ncomms10116

Figure 3. Lsd1 KO oocytes resume meiosis due to abnormal activation of CDK1.

Figure 3

(a) Western blot analysis of LSD1, phospho-Tyr15-CDK1 (pY15-CDK1), CDK1 and cyclin B1. α-tubulin serves as a loading control. Each lane contained 100 fully grown GV oocytes. Full blots are provided in Supplementary Fig. 9. (b) Fully grown GV oocytes were collected in medium containing IBMX and, following IBMX washout, the oocytes were cultured in the presence or absence of the CDK1 inhibitor roscovitine (ROS, 50 μM) for 1 h to determine GVBD rates. The data represent the mean±s.e.m. of three experiments. Statistical comparisons of values were made using one-way ANOVA. **P<0.01.