Figure 3. Organoid morphology and phenotype remains stable during long-term culture.

(a) Comparative confocal analysis of the organoids after 1 and 4 months in vitro reveals stability of the phenotype, with unchanged morphology and staining pattern of the adhesion markers. (b) Dynamics of organoid growth remains stable over time as determined by quantification of the relative number of proliferative Ki-67-positive cells in confocal sections at 1- and 8-month culture. (c) The proportion of ciliated cells also remains unchanged as determined by quantification of ciliated cells. Error bars represent±s.d. from a minimum of five independent fields of view.