Figure 6. Notch signalling is required for maintaining a secretory phenotype.

Representative confocal images of control (a) and DBZ-treated (1 μM) organoids (b), showing an increase in the number of ciliated cells positive for acetylated tubulin (green), as well as a decrease in the number of proliferating cells positive for Ki67 (red) when Notch is inhibited. (c,d) Quantification of ciliated and actively proliferating cells in control versus DBZ-treated organoid sections from three different donors. Error bars represent±s.d. from a minimum of seven independent fields of view. (e) Organoids respond to oestradiol (500 pmol l⁻¹) and progesterone (50 ng ml⁻¹) stimulation as determined by gene expression profiling by microarray analysis of three independent donors. The colour matrix of the heat map depicts the log10(Ratio) of individual unstimulated (C) versus hormone-stimulated (estradiol, E or progesterone, P) comparisons while the hierarchical structures are illustrated as dendrograms at gene and patient levels.