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. 2015 Dec 22;5:18478. doi: 10.1038/srep18478

Figure 4. Characterization of the original and remodeled unnatural-base DNA aptamers.

Figure 4

(a) Original and (b–d) remodeled anti-IFNγ DNA aptamers (a) aptamer 49, (b) aptamer 58, (c) aptamer 57 mh, (d) aptamer 57 mhGC), characterized by nuclease resistance in 96% human serum at 37 °C (Supplementary Fig. 3), thermal stability (Supplementary Fig. 2), binding ability analyzed by BIAcore T200 in the presence of 1.25–50 nM IFNγ (Supplementary Fig. 5), and STAT1 phosphorylation for the inhibition of IFNγ (FACS analysis). Black and red peaks represent human IFNγ treatment with and without aptamers, respectively. Grey peaks represent unstimulated cells.