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. 2015 Dec 22;6:1428. doi: 10.3389/fmicb.2015.01428

FIGURE 4.

FIGURE 4

Three-dimensional projections or single layers of CLSM images of Lm EGD-e flow chamber biofilms grown for 24 h under hydrodynamic conditions at 37°C in 0.1BHI (A) or BHI (B) or at 25°C in 0.1BHI (C) and (D). Live bacteria are stained by SYTO-60 (red) and eDNA with TOTO-1 (green). (D) Digital magnification of eDNA spots identified in the layer of the z-stacks that corresponds to the surface of the slide (size bar indicates 10 μm).