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. 2015 Dec 22;5:18638. doi: 10.1038/srep18638

Figure 6. Predicted peptide p_wt14 inhibit CXCL8 and LPS induced monocytes adhesion and transmigration.

Figure 6

(A) p_wt14 inhibits CXCL8 induced monocytes transmigration. HMEC-1 was pretreated with various concentrations of p_wt14 for one hour and then combined with 25 ng/ml CXCL8 for 18 hours of incubation, before the THP-1 cells were allowed to transmigrate through the HMEC-1 monolayer. The transendothelial migrated monocytes were measured and calculated by counting cells migrating to the bottom wells under microscopy. (B) p_wt14 inhibits LPS induced monocytes adhesion. (C) p_wt14 inhibits LPS induced transmigration. HMEC-1 was pretreated with various concentrations of p_wt14 for one hour and then combined with 25 ng/ml LPS for 18 hours of incubation, before the THP-1 were allowed to adhesion to HMEC-1 (B) or transmigrate through the HMEC-1 monolayer (C). Values are mean ± SD from three independent experiments. (*P < 0.05) as compared to control; (#P < 0.05) as compared to cells stimulated with CXCL8 or LPS in the absence of p_wt14. (D) p_wt14 does not induce the cytotoxic effect on endothelial cells. HMEC-1 was treated with various concentrations of p_wt14 in 96-well plate. After 24 and 48 hours of incubation, cell viability was evaluated using the colorimetric WST-1 assay. Data are the mean ± SD of triplicate determinations. “Control” means that only the culture medium (without peptides) is incubated with cells.