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. 2015 Dec 21;10(12):e0144803. doi: 10.1371/journal.pone.0144803

Fig 1. Effects of preconditioning conditions on anhydrobiotic survival in Z151 strain of H. dujardini.

Fig 1

(A) Experimental scheme of the desiccation tolerance assay. Tardigrades in aqueous fluid were dropped onto a nylon filter placed on filter paper, and preconditioned at 95% RH for 0 to 4 days. After preconditioning, they were desiccated at 10% RH for 2 days and humidified at 95% RH for 1 day prior to rehydration. Recovery rates were examined at 1 h after rehydration. (B, C) Effects of various preconditioning periods on anhydrobiotic survival at 1h (B) and 24h (C). Data are shown as mean ± SD (N = 4; 20 tardigrades each). (D, E) Effects of relative humidity exposure during preconditioning on anhydrobiotic survival at 1h (D) and 24h (E). The preconditioning period was fixed as 1 day, and tardigrades were exposed to 33.5%, 62%, 85%, or 95% RH as preconditioning. Data are shown as mean ± SD (N = 4; 20 tardigrades each).