Table 1. Protein identification from nano-liquid chromatography-electrospray-ion trap-tandem mass spectrometry analysis.
| Spotnumber | Protein ID | Accessionnumber | Genename | Mw(Da) | Peptide sequence | Start-Endsequence | P (pep) | z | Peptidemass (Da) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Glutenin, high molecular weight subunit PW212 | P08489 | GLT4 | 89119.8 | K.GGSFYPGETTPPQQLQQR.I | 82–99 | 1.73E-09 | 2 | 1990.9617 |
| K.GGSFYPGETTPPQQLQ*QR.I | 1.54E-04 | 2 | 2048.0322 | ||||||
| Alpha/beta-gliadin | P02863 | GDA0 | 32942.7 | R.PSQQNPQAQGSVQPQQLPQFEEIR.N | 233–256 | 2.35E-08 | 3 | 2734.3543 | |
| Alpha-amylase inhibitor 0.19 | P01085 | IAA1 | 13328.4 | R.LQ*CNGSQVPEAVLR.D | 26–39 | 2.18E-06 | 2 | 1570.8496 | |
| R.LQCNGSQ*VPEAVLR.D | 3.27E-05 | 2 | 1570.8496 | ||||||
| Alpha-amylase/trypsin inhibitor CM3 | P17314 | IAAC3 | 18209.1 | R.DYVLQQ*TCGTFTPGSK.L | 45–60 | 4.69E-06 | 2 | 1801.8915 | |
| R.DYVLQ*QTCGTFTPGSK.L | 7.33E-04 | ||||||||
| 2 | Glutenin, high molecular weight subunit DY10 | P10387 | GLT0 | 69586.7 | K.GQQGYYPTSLQQPGQGQQGYYPTSLQHTGQR.Q | 185–215 | 1.89E-13 | 3 | 3453.6207 |
| Gamma-gliadin | P08453 | GDB2 | 37098.8 | R.PFIQPSLQQQ*LNPCK.N | 171–185 | 2.63E-05 | 2 | 1797.9806 | |
| R.PFIQPSLQQ*QLNPCK.N | 3.05E-04 | ||||||||
| 3 | Beta-amylase | P93594 | AMYB | 56575.2 | R.NIEYLTLGVDDQPLFHGR.T | 129–146 | 8.54E-12 | 2 | 2087.0556 |
| 4 | Alpha-amylase/trypsin inhibitor CM3 | P17314 | IAAC3 | 18209.1 | R.DYVLQQ*TCGTFTPGSK.L | 45–60 | 1.17E-05 | 2 | 1801.8915 |
| R.DYVLQ*QTCGTFTPGSK.L | 1.42E-04 | ||||||||
| R.LLVAPGQ*CNLATIHNVR.Y | 141–157 | 5.71E-05 | 3 | 1876.0711 | |||||
| Alpha-amylase inhibitor 0.19 | P01085 | IAA1 | 13328.4 | R.LQ*CNGSQVPEAVLR.D | 26–39 | 8.06E-05 | 2 | 1570.8496 | |
| R.LQCNGSQ*VPEAVLR.D | 9.94E-04 | 1570.8496 | |||||||
| 5 | Serpin-Z1A | Q41593 | SPZ1A | 43091.2 | K.LVLANALYFK.G | 172–181 | 5.14E-08 | 2 | 1151.6823 |
| K.LSAEPDFLER.H | 261–270 | 5.30E-08 | 2 | 1176.5895 | |||||
| K.AAEVTTQVNSWVEK.V | 138–151 | 5.15E-07 | 2 | 1561.7857 | |||||
| K.ISFGIEASDLLK.C | 189–300 | 2.59E-06 | 2 | 1292.7096 | |||||
| K.LSAEPDFLER.H | 261–270 | 9.06E-05 | 1 | 1176.5895 | |||||
| 6 | Alpha-amylase inhibitor 0.19 | P01085 | IAA1 | 13328.4 | R.LQ*CNGSQVPEAVLR.D | 26–39 | 3.10E-06 | 2 | 1570.8496 |
| R.LQCNGSQ*VPEAVLR.D | 5.12E-04 | ||||||||
| Gamma-gliadin | P08453 | GDB2 | 37098.8 | R.PFIQPSLQQQ*LNPCK.N | 171–185 | 1.21E-05 | 2 | 1797.9806 | |
| R.PFIQPSLQQ*QLNPCK.N | 1.67E-04 | ||||||||
| R.APFASIVAGIGGQ | 315–327 | 9.62E-04 | 1 | 1187.6419 | |||||
| 7 | Alpha/beta-gliadin | P02863 | GDA0 | 32942.7 | R.PSQQNPQAQGSVQPQQLPQFEEIR.N | 233–256 | 1.67E-14 | 2 | 2734.3543 |
| R.PSQQNPQAQ*GSVQPQQLPQFEEIR.N | 4.44E-07 | 2 | 2791.4248 | ||||||
| R.PSQQNPQ*AQGSVQPQQLPQFEEIR.N | 4.17E-05 | 2 | |||||||
| R.PSQQNPQAQ*GSVQPQQLPQFEEIR.N | 2.31E-04 | 3 | |||||||
| R.PSQQ*NPQAQGSVQPQQLPQFEEIR.N | 3.46E-04 | 2 | |||||||
| R.PSQ*QNPQAQGSVQPQQLPQFEEIR.N | 3.46E-04 | 2 | |||||||
| R.PSQQNPQAQGSVQ*PQQLPQFEEIR.N | 3.84E-04 | 2 | |||||||
| Gamma-gliadin | P08453 | GDB2 | 37098.8 | R.PFIQPSLQQQ*LNPCK.N | 171–185 | 6.44E-05 | 2 | 1797.9806 | |
| R.PFIQPSLQQQ*LNPCK.N | 6.42E-04 | 3 | |||||||
| R.PFIQPSLQQ*QLNPCK.N | 6.64E-04 | 2 | |||||||
| 8 | Alpha/beta-gliadin | P02863 | GDA0 | 32942.7 | R.PSQQNPQAQGSVQPQQLPQFEEIR.N | 233–256 | 3.07E-08 | 2 | 2734.3543 |
| R.PSQQNPQAQ*GSVQPQQLPQFEEIR.N | 4.25E-07 | 2 | 2791.4248 | ||||||
| R.PSQQNPQ*AQGSVQPQQLPQFEEIR.N | 5.04E-05 | ||||||||
| R.PSQ*QNPQAQGSVQPQQLPQFEEIR.N | 4.35E-04 | ||||||||
| R.PSQQ*NPQAQGSVQPQQLPQFEEIR.N | 4.35E-04 | ||||||||
| Gamma-gliadin | P08453 | GDB2 | 37098.8 | R.APFASIVAGIGGQ | 315–327 | 3.49E-05 | 2 | 1187.6419 | |
| R.PFIQPSLQQQ*LNPCK.N | 171–185 | 5.53E-04 | 2 | 1797.9806 |
Q* γ-glutamyl-n-butylamine.