Skip to main content
. 2015 Dec 22;5:18687. doi: 10.1038/srep18687

Figure 3. Effects of 1,25(OH)2D3 on LPS-induced inflammatory cytokines and chemokines in human HK-2 cells.

Figure 3

Human HK-2 cells were transfected with either pooled VDR siRNA (100 nM) or random siRNA (100 nM, as control) as Materials and Methods. Human HK-2 cells were then cultured with LPS (5.0 μg/ml) for 2 h in presence or absence of 1,25-(OH)2D3 (100 nM). TNF-α, IL-1β, IL-8 and MCP-1 mRNAs were measured using real-time RT-PCR. VDR siRNA (–) denotes the pretreatment with random siRNA as control. All data were expressed as means ± S.E.M (n = 6). *P < 0.05, **P < 0.01.