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. 2015 Dec 22;5:18687. doi: 10.1038/srep18687

Figure 7. The interaction between renal VDR and NF-κB p65.

Figure 7

In VitD3 + LPS group, mice were pretreated with three doses of VitD3 (25 μg/kg) at 48, 24 and 1 h before LPS (1.0 mg/kg) injection. Kidney samples were collected 1 h after LPS injection. The nuclear fractions were prepared from kidneys and incubated with agarose-conjugated either VDR or NF-κB p65 antibody. NF-κB p65 and VDR were measured using immunoblots. All experiments were duplicated for four times. All data were expressed as means ± S.E.M. (n = 4). **P < 0.01.