Skip to main content
. 2015 Oct 8;65(1):149–163. doi: 10.2337/db15-0108

Figure 2.

Figure 2

miR-200b interacts with CITED2 mRNA. A: Schematic representation of the CITED2 mRNA depicting the two miR-200b binding sites (BS) in its 3′-UTR. Alignment of the CITED2 mRNA sequence with miR-200b: top strand, CITED2 mRNA; bottom strand, miR-200b. B: miR-200b levels and U6 RNA levels after 48-h biotinylated miR-200b transfection. Bio-con, the biotin-labeled negative control; Bio-200b, biotin-labeled miR-200b. C: CITED2 mRNA levels in the materials pulled down by biotin–miR-200b and levels of total input CITED2 mRNA. D: Changes in CITED2 mRNA levels in biotin–miR-200b pulldown after addition of unlabeled synthetic RNA sense strands (RSS1 and RSS2) against CITED2 3′-UTR (positions from 1514 to 1536 and 1846 to 1867, encompassing the two miR-200b binding sites) to the reaction buffer in the RNA pulldown assay. Experiments were repeated three times (n = 3). *Significant differences (P < 0.05) compared with the control group or the other two groups.