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. 2015 Dec 21;10(12):e0145538. doi: 10.1371/journal.pone.0145538

Fig 6. Effect of bitter agonists on differentiation of 3T3-F442A preadipocytes.

Fig 6

(a) Representative Oil-Red O stained 3T3-F442A cells at day 12 of differentiation in the presence of vehicle, 150 μM DB or 100 μM Q. (b) Quantification of the Oil-Red O uptake at differentiation day 6 (n = 4) and 12 (n = 8). (c-d) Relative mRNA expression of the markers leptin and adiponectin during differentiation in the absence and presence of DB or Q (n = 6). (e) Trypan blue cell viability assay, presented as the amount of dead cells (% cells stained) after the 12-day differentiation period in the absence and presence of DB or Q (n = 3). **: P<0.01, ***: P<0.001 vehicle vs bitter.