Table 1.
Effects of chronic cocaine, heroin and speedball self-administration on parameters of high- and low-affinity DAT binding sites.
| SA Group | High Kd (pM) | Low Kd (pM) | High Bmax (pmol/mg) | Low Bmax (pmol/mg) |
|---|---|---|---|---|
|
| ||||
| Saline | 132.6 ± 46.1 | 16880 ± 2938 | 0.286 ± 0.140 | 4.561 ± 1.340 |
| Cocaine | 410.3 ± 98.2 | 7960 ± 2190* | 0.284 ± 0.095 | 3.035 ± 0.470 |
| Speedball | 255.6 ± 81.9 | 4993 ± 1256* | 0.994 ± 0.355 | 5.878 ± 0.815 |
| Heroin | 237.3 ± 22.7 | 11442 ± 1774 | 0.228 ± 0.083 | 3.011 ± 1.159 |
Binding parameters for high-affinity (high Kd and Bmax) and low-affinity (low Kd and Bmax) DAT binding sites (mean ± SEM) determined by nonlinear regression for two-site modeling of membrane binding assays with [125I]RTI-55 in rat NAc membranes.
*
p < 0.05 vs. saline.