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. Author manuscript; available in PMC: 2015 Dec 22.
Published in final edited form as: Methods Mol Biol. 2012;905:201–211. doi: 10.1007/978-1-61779-949-5_12

Fig. 2.

Fig. 2

Gel mobility shift analysis of TRAP-trp leader RNA interaction. 5′ end-labeled trp leader RNA (0.1 nM) was incubated with the concentration of TRAP shown at the top of each lane. Samples were loaded onto 6% polyacrylamide gels with a 37.5:1 acrylamide:bisacyrylamide ratio. Positions of bound and free RNA are shown. (a) TBE buffering system. (b) Tris-glycine buffering system. The Tris-glycine buffering system gave superior results in this particular case. The Kd for this reaction was calculated to be 17 nM TRAP.