Figure 2. Microbleeds in cerebral cortex of EFAD mice: female excess is additive with APOE4.
A. Microbleeds visualized as extravasated hemosiderin by Prussian blue histochemistry in cerebral cortex sagital sections (0.5–2 mm lateral from midline; counterstained by nuclear fast red; scale bar 50 µm). The inset shows cortical layers I and III. B. Number of microbleeds per 100 mm2 of cortical area for EFAD mice. Female excess was observed in both EFAD mice. C. Hemosiderin load (total Prussian blue area, %) showed female excess and additivity with APOE4 in EFAD. * p<0.05; ** p <0.01; **** p<0.001. Male microbleeds did not differ by APOE allele.