Fig 6. A Nonsynonymous/Synonymous Analysis of the B56 Gene Family, and B56-1 and B56-2 Subfamilies, at the Single Amino Acid Level.

dN/dS values were calculated for each amino acid, and the average was plotted with 95% confidence interval brackets for each position in which ω was not undefined. Sample sets included the entire gene family (all: B56α, B56β, B56γ, B56δ, and B56ε), and the B56 subfamilies (B56-1 and B56-2). Dashed lines demarcate the core domain. The data were superimposed on a pictorial representation of the structure of the B56 conserved core domain with each number above a lightly hued bar denoting a pseudo-HEAT repeat and the darker bars within those representing α-helices. Amino acid positions in which dN/dS calculations generated an undefined number (dS equal to zero) that therefore could not be graphed are denoted by red circles if dN equaled zero, or green squares if dN was any number but zero.