Skip to main content
. 2015 Dec 22;5:18657. doi: 10.1038/srep18657

Figure 4. Knockdown of PRKDC and CPQ-PRKDC by siRNA.

Figure 4

(a) Schematics of CPQ-PRKDC fusion transcripts and wild-type PRKDC. PRKDC-specific, fusion-specific and dual siRNAs were used in the knockdown experiments. Expression levels of the wild-type PRKDC (PCR product 1, beige), dual PRKDC (PCR product 2, green) and CPQ-PRKDC (PCR product 3, pink) were measured using appropriate primers. (b,c) PRKDC siRNAs were transfected to JHUEM3 harboring CPQ-PRKDC fusion transcript. The mRNA expressions of CPQ-PRKDC and dual PRKDC were assessed by quantitative real-time RT-PCR. Data represent the mean and error bar of triplicate independent experiments (asterisk denotes p < 0.01). (d) The effect of siRNA-mediated down-regulation of PRKDC on JHUEM3 cell proliferation. The cell proliferation was assessed by CellTiter-Glo assay. Data represent the mean and error bar of quadruplicate independent experiments (asterisk denotes p < 0.01). (e,f) Fusion-specific siRNA were transfected into JHUEM3 harboring an CPQ-PRKDC fusion. The mRNA expression levels of CPQ-PRKDC and wild-type PRKDC were assessed by quantitative real-time RT-PCR. Data represent the mean and error bar of triplicate independent experiments (asterisk denotes p < 0.01). (g) The effect of siRNA-mediated down-regulation of CPQ-PRKDC on JHUEM3 cell proliferation.